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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
19/09/2014 |
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Actualizado : |
23/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ALEM, D.; NARANCIO, R.; DIAZ, P.; REBUFFO, M.; ZARZA, R.; DALLA RIZZA, M. |
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Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RAFAEL NARANCIO FERES, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MONICA IRENE REBUFFO GFELLER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RODRIGO TABARE ZARZA FUENTES, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers. |
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Fecha de publicación : |
2011 |
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Fuente / Imprenta : |
Ciencia e Investigación Agraria, Santiago de Chile, v. 38, n. 3, p. 463-471, 2011. |
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ISSN : |
0304-5609 |
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DOI : |
10.4067/S0718-16202011000300015 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received September 7 2010. /Accepted June 21, 2011. |
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Contenido : |
Abstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in breeding programs. MenosAbstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in b... Presentar Todo |
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Palabras claves : |
GENETIC VARIABILITY; LOTUS JAPONICUS; MICROSATELLITES; SIMPLE SEQUENCE REPEATS; SSR; VARIABILIDAD GENÉTICA. |
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Thesagro : |
CULTIVOS. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.scielo.cl/pdf/ciagr/v38n3/art15.pdf
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Marc : |
LEADER 02470naa a2200301 a 4500
001 1050403
005 2019-10-23
008 2011 bl uuuu u00u1 u #d
022 $a0304-5609
024 7 $a10.4067/S0718-16202011000300015$2DOI
100 1 $aALEM, D.
245 $aMolecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers.$h[electronic resource]
260 $c2011
500 $aArticle history: Received September 7 2010. /Accepted June 21, 2011.
520 $aAbstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in breeding programs.
650 $aCULTIVOS
653 $aGENETIC VARIABILITY
653 $aLOTUS JAPONICUS
653 $aMICROSATELLITES
653 $aSIMPLE SEQUENCE REPEATS
653 $aSSR
653 $aVARIABILIDAD GENÉTICA
700 1 $aNARANCIO, R.
700 1 $aDIAZ, P.
700 1 $aREBUFFO, M.
700 1 $aZARZA, R.
700 1 $aDALLA RIZZA, M.
773 $tCiencia e Investigación Agraria, Santiago de Chile$gv. 38, n. 3, p. 463-471, 2011.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
16/12/2019 |
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Actualizado : |
17/12/2019 |
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Tipo de producción científica : |
Documentos |
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Autor : |
LADO, J.; PINTOS, P.; LUQUE, E.; MOLTINI, A.; GAMBETTA, G.; SALVO, M.; ARRUABARRENA, A. |
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Afiliación : |
JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PEDRO MAXIMILIANO PINTOS RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MAYZA ELEANA LUQUE NUÑEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA INÉS MOLTINI PALADINO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GIULIANA GAMBETTA, Departamento de Producción Vegetal, Facultad de Agronomía, Universidad de la República.; MATÍAS SALVO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Aplicación de bajas temperaturas en la postcosecha de los frutos cítricos: factores involucrados en la tolerancia al daño por frío. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Frutícola. Resultados de Investigación en Postcosecha de Frutos Cítricos. Jornada de divulgación. INIA Salto Grande, 29 de Noviembre de 2019. Salto Grande (UY): INIA. |
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Páginas : |
p. 2-12. |
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Serie : |
(Serie Actividades de Difusión; 794). |
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ISSN : |
1688-9258 |
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Idioma : |
Español |
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Contenido : |
A continuación, se presenta un resumen de los principales resultados obtenidos en relación con el estudio de los factores que podrían influir en el desarrollo de tolerancia al daño por frío postcosecha y también un abordaje de los posibles mecanismos endógenos que regulan este proceso de respuesta. |
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Palabras claves : |
TOLERANCIA AL FRÍO. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13917/1/SAD-794-Lado-J.-et-al..pdf
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Marc : |
LEADER 01241nam a2200229 a 4500
001 1060537
005 2019-12-17
008 2019 bl uuuu u0uu1 u #d
022 $a1688-9258
100 1 $aLADO, J.
245 $aAplicación de bajas temperaturas en la postcosecha de los frutos cítricos$bfactores involucrados en la tolerancia al daño por frío.$h[electronic resource]
260 $aIn: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Frutícola. Resultados de Investigación en Postcosecha de Frutos Cítricos. Jornada de divulgación. INIA Salto Grande, 29 de Noviembre de 2019. Salto Grande (UY): INIA.$c2019
300 $ap. 2-12.
490 $a(Serie Actividades de Difusión; 794).
520 $aA continuación, se presenta un resumen de los principales resultados obtenidos en relación con el estudio de los factores que podrían influir en el desarrollo de tolerancia al daño por frío postcosecha y también un abordaje de los posibles mecanismos endógenos que regulan este proceso de respuesta.
653 $aTOLERANCIA AL FRÍO
700 1 $aPINTOS, P.
700 1 $aLUQUE, E.
700 1 $aMOLTINI, A.
700 1 $aGAMBETTA, G.
700 1 $aSALVO, M.
700 1 $aARRUABARRENA, A.
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